| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lord, Madison (9)
|
MRBAD |
34.67 |
35.16 |
0.49 |
| 2 |
Hartnagel, Olivia R (9)
|
MRTVSC |
38.11 |
35.97 |
-2.14 |
| 3 |
Chu, Marissa (9)
|
MRPAC |
37.08 |
36.09 |
-0.99 |
| 4 |
Gohman, Sullivan W (9)
|
MRBAD |
37.30 |
36.70 |
-0.60 |
| 5 |
Augustensen, Isabella M (8)
|
MRPAC |
38.40 |
37.20 |
-1.20 |
| 6 |
Tieng, Alexandra K (8)
|
MRBAD |
39.30 |
37.70 |
-1.60 |
| 7 |
Czaja, Julia O (9)
|
MRLGAC |
37.28 |
37.73 |
0.45 |
| 8 |
Emmerich, Peyton M (9)
|
MRTVSC |
39.33 |
37.75 |
-1.58 |
| 9 |
Dassen, Arwen M (9)
|
MRTVSC |
38.03 |
38.09 |
0.06 |
| 10 |
Chan, Melanie Y (9)
|
MRLIAC |
39.17 |
38.56 |
-0.61 |
| 11 |
Jukic, Mia A (9)
|
MRAGUA |
38.99 |
38.58 |
-0.41 |
| 12 |
Ringh, Anna V (9)
|
MRLIAC |
37.99 |
38.67 |
0.68 |
| 13 |
Somarriba, Gracie E (9)
|
MRCOND |
37.75 |
38.77 |
1.02 |
| 14 |
Xiao, Queenie (9)
|
MRTRI |
38.50 |
39.05 |
0.55 |
| 15 |
Vater, Annabelle A (9)
|
MRBKR |
39.06 |
40.00 |
0.94 |
| 16 |
Erb, Chloe F (9)
|
MRBAD |
39.02 |
40.12 |
1.10 |
| 17 |
Muety, Maeve K (8)
|
MRLIAC |
38.77 |
40.75 |
1.98 |
| 18 |
Vargas, Izabella C (9)
|
MRTVSC |
39.30 |
40.78 |
1.48 |
| 19 |
Kim, Nadia J (9)
|
MRLIAC |
39.15 |
41.14 |
1.99 |
| 20 |
DiBenedetto, Milania A (9)
|
MRPATS |
49.96 |
44.09 |
-5.87 |
|
la Fleur, Grace C (9)
|
MRBGNW |
38.61 |
NS |
- |
|
Dorjee, Ngawang C (9)
|
MRLGAC |
39.35 |
NS |
- |